講演詳細

遺伝・育種部門[Forest Genetics and Tree Breeding]

日付	2026年3月17日
開始時刻	ポスター発表
会場名	多目的ホール
講演番号	PF-16
発表題目	Haplotype-resolved chromosome-level genome assembly in Abies sachalinensis Haplotype-resolved chromosome-level genome assembly in Abies sachalinensis
所属	東京大学
要旨本文	High repeat content and heterozygous conifer genomes remain challenging to assemble. Here, we report a haplotype-resolved chromosome-level genome of Sakhalin fir (Abies sachalinensis), one of the major species for silviculture and ecosystem in northern Japan. Using HiFi sequencing and RNA-seq, we assembled two haplotype genomes (HapA 15.91Gbp, HapB 15.78Gbp) and annotated complete protein-coding genes (HapA 46,145, HapB 46,116). The assemblies show high contiguity and completeness (BUSCO: HapA 90.5%, HapB 90.5%), and reveal structural differences between haplotypes. High repeat content (HapA 82.77%, HapB 82.92%) was detected in both haplotypes. This haplotype-resolved chromosome-level genome provides an allelic view of gene content, structural variation, and repeat content. The results can be applied in local adaptation and genome evolution in Abies and related conifers.
著者氏名	○Xinjie Cai1 ・ Shuji Shigenobu2 ・ Katsushi Yamaguchi2 ・ Masahiro Kasahara3 ・ Takeshi Fujino3 ・ Wataru Ishizuka4 ・ Kentaro Uchiyama5 ・ Yoko Hisamoto1 ・ Keiko Kitamura6 ・ Susumu Goto1
著者所属	1Graduate School of Agricultural and Life Sciences, The University of Tokyo ・ 2National Institute for Basic Biology ・ 3Graduate School of Frontier Sciences, The University of Tokyo ・ 4Forest Research Department, Forest Research Institute, Hokkaido Research Organization ・ 5Forestry and Forest Products Research Institute ・ 6Hokkaido Research Center, Forestry and Forest Products Research Institute
キーワード	Haplotype-resolved, chromosome-level, genome assembly, Abies sachalinensis
Key word	Haplotype-resolved, chromosome-level, genome assembly, Abies sachalinensis