第135回日本森林学会大会 発表検索

講演詳細

微生物部門[Forest Microbes]

日付 2024年3月8日
開始時刻 ポスター発表
会場名 543
講演番号 PM-6(学生ポスター賞審査対象)
発表題目 Analysis of related gene expression and genotype frequency underlying ectomycorrhizal formation in Cenococcum geophilum
Analysis of related gene expression and genotype frequency underlying ectomycorrhizal formation in Cenococcum geophilum
所属 東京大学
要旨本文 Cenococcum geophilum (Cg) thrives in global forests, establishing symbiotic relationships with trees. However, the molecular mechanism of mycorrhizal formation remains unclear. Through a gene-wide association study (GWAS), 1107 genes and 105 significant SNPs were identified. Twenty genes were selected for further analysis combined with the transcriptomic analysis. Notably, a positive correlation between the mutation rate among 105 SNPs and Cg infectivity was observed. Subsequently, 10 highly infectious Cg isolates with elevated mutation rates and 10 non-infectious isolates with low mutation rates underwent a one-month inoculation experiment on Populus tomentosa to quantify the gene expressions by RT-qPCR. Additionally, the distribution of different genotypes in the Cg population revealed a significant positive correlation with infectivity. Overall, this study provides insight into the relationship between the infectivity and genotype of Cg.
著者氏名 ○Kong, Dening ・ Matsushita, Norihisa ・ Okabe, shin ・ Lian, Chunlan
著者所属 The University of Tokyo
キーワード Cenococcum geophilum, genotype, ectomycorrhizal formation
Key word Cenococcum geophilum, genotype, ectomycorrhizal formation